Gene name |
SPAC458.03 |
Gene ID |
38/F04 |
Gene synonyms/obsolete |
|
Gene product |
leucine-rich repeat
protein; involved in telomere maintenance; involved in
telomerase-dependent telomere maintenance |
Entry clone |
Cloned |
ORF length (unspliced) |
2607 |
ORF length (spliced) |
|
Entry clone length |
2607 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
2288T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC458.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAATCCTTTGCTAGCGA |
Rev primer name |
SPAC458.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGTCCGGCTAATCCAAAT |
Amino acid length |
868 |
Molecular weight |
99 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAKCFSTLLGL/LSLKVDKSLFL/LVYYLKTLGI/LLFSFLAILEI/LSEFRTLAL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
Microscope used for
observation |
Leica |