| Gene name |
SPCC330.02 |
| Gene ID |
35/H08 |
| Gene synonyms/obsolete |
SPCC613.14 |
| Gene product |
leucine-rich repeat
protein; involved in DNA repair and nucleotide-excision
repair |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1740 |
| ORF length (spliced) |
1692 |
| Entry clone length |
1740 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC330.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAGTGGAAGTCGGGT |
| Rev primer name |
SPCC330.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGAACTTCACGCCCTATC |
| Amino acid length |
563 |
| Molecular weight |
62.7 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
164 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB?; nuclear dots;
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |