| Gene name |
SPCC1672.05c |
| Gene ID |
34/E02 |
| Gene synonyms/obsolete |
|
| Gene product |
tyrosine-tRNA ligase;
involved intyrosyl-tRNA aminoacylation; tyrosine-tRNA ligase
activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1206 |
| ORF length (spliced) |
|
| Entry clone length |
1206 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
852A:G / 950T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1672.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGCTTACTCCGGATGA |
| Rev primer name |
SPCC1672.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCATCCAGATTCAATTTT |
| Amino acid length |
401 |
| Molecular weight |
44.5 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol (intranuclear microtubule
bundle?) |
| Microscope used for
observation |
Confocal |