| Gene name |
SPBC4C3.02c |
| Gene ID |
33/G10 |
| Gene synonyms/obsolete |
ade1; SPBC405.01 |
| Gene product |
phosphoribosylamine-glycine ligase;
phosphoribosylformylglycinamidine cyclo-ligase; involved in
purine biosynthesis; bifunctional enzyme; de novo purine
biosynthesis pathway (2nd step); de novo purine biosynthesis
pathway (5th step) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2367 |
| ORF length (spliced) |
|
| Entry clone length |
2367 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
78T:C / 138A:G /
743A:G / 1710T:C / 2246A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC4C3.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACCCATTATTGCGTT |
| Rev primer name |
SPBC4C3.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAATGTTTCCCATTTGTTC |
| Amino acid length |
788 |
| Molecular weight |
85.2 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVGILFTGLML |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |