| Gene name |
SPBC15D4.04 |
| Gene ID |
32/H04 |
| Gene synonyms/obsolete |
gpt2; gpt |
| Gene product |
UDP-N-acetylglucosamine--dolichyl-phosphate
N-acetylglucosaminephosphotransferase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1341 |
| ORF length (spliced) |
|
| Entry clone length |
1341 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1005T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC15D4.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATAGAGTCCTGTTTTAA |
| Rev primer name |
SPBC15D4.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATATGTTATCTTCAGGATAC |
| Amino acid length |
446 |
| Molecular weight |
49.8 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
9 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGAYLSALLSI/LRAHLLSLYL/LGHFSKTLML/LTISIFEKLRL/LTICIMGLQL/LTGIFGLII |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |