| Gene name |
SPBC17A3.05c |
| Gene ID |
32/H03 |
| Gene synonyms/obsolete |
pi041 |
| Gene product |
DNAJ domain protein;
no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1330 |
| ORF length (spliced) |
1212 |
| Entry clone length |
1330 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
222A:G / 420A:G /
748T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC17A3.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATCTAGTAGAGAAGA |
| Rev primer name |
SPBC17A3.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAATAATAACTGTTTGAA |
| Amino acid length |
403 |
| Molecular weight |
46.3 |
| Isoelectric point (calc.) |
9.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |