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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6F6.15
Gene ID 32/D12
Gene synonyms/obsolete ypt5
Gene product small GTPase; Rab subfamily; GTP-binding protein; post-translational modification, gerenylgerenylation @ C-terminal CXC; post-translational modification, carboxymethylation; involved in regulation of endosome fusion, the regulation of intracellular protein transport; functionally complemented by mammalian rab5; non-essential
Entry clone Cloned
ORF length (unspliced) 1089
ORF length (spliced) 636
Entry clone length 1089
No. of intron 7
Sequence status Finished
Sequence results 36A:G / 493G:A / 712A:G / 878A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC6F6.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCATCAAATACAGCTCC
Rev primer name SPAC6F6.15.Rv
Rev primer SEQ AGAAAGCTGGGTAGCAAGAGCATGAACCGCTT
Amino acid length 211
Molecular weight 22.9
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFTAIAKKLPL
Localization (YFP) cytosol=nucleus
Comments for localization bright signal
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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