| Gene name |
SPBC409.05 |
| Gene ID |
31/G07 |
| Gene synonyms/obsolete |
psh1; skp1; sph1 |
| Gene product |
SCF ubiquitin ligase
complex; complexes with Pop1p and Pop2p; essential; involved
in cytokinesis and cell separation; skp1 family |
| Entry clone |
Cloned |
| ORF length (unspliced) |
658 |
| ORF length (spliced) |
486 |
| Entry clone length |
658 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC409.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCAAAATCAAACTGAT |
| Rev primer name |
SPBC409.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCTGTCTTCGGCCCATTCC |
| Amino acid length |
161 |
| Molecular weight |
18.7 |
| Isoelectric point (calc.) |
4.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |