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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1685.09
Gene ID 31/C04
Gene synonyms/obsolete rps29
Gene product 40S ribosomal protein S29; non-essential
Entry clone Cloned
ORF length (unspliced) 294
ORF length (spliced) 171
Entry clone length 294
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1685.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCCCACGAAAACGTTTG
Rev primer name SPBC1685.09.Rv
Rev primer SEQ AGAAAGCTGGGTAACGGTACTAAACTAGTTAA
Amino acid length 56
Molecular weight 6.6
Isoelectric point (calc.) 11.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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