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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1494.07
Gene ID 30/H08
Gene synonyms/obsolete
Gene product conserved hypothetical protein
Entry clone Cloned
ORF length (unspliced) 4509
ORF length (spliced)
Entry clone length 4509
No. of intron 0
Sequence status Finished
Sequence results 1173A:G / 3646T:C / 4233T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1494.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTGAGTGTTATCAA
Rev primer name SPCC1494.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTGACAAAAGCTAGGATGT
Amino acid length 1502
Molecular weight 171.1
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTVLLTKCLSL/LSCFFSKLTL
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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