| Gene name |
SPBC27.04 |
| Gene ID |
30/B07 |
| Gene synonyms/obsolete |
|
| Gene product |
predicted coiled-coil
protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3159 |
| ORF length (spliced) |
|
| Entry clone length |
3159 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
545T:C / 1216T:C /
1440A:G / 2854G:T / 2907T:C / 3055A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC27.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGTTTCATGGATGTTT |
| Rev primer name |
SPBC27.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCTGCAGAAGTAGATACT |
| Amino acid length |
1052 |
| Molecular weight |
118.2 |
| Isoelectric point (calc.) |
4.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVERMSRILEI/LLEQVRHLEL |
| Localization (YFP) |
cytosol; periphery at
cell tip and site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |