| Gene name |
SPAC22H10.01c |
| Gene ID |
30/B06 |
| Gene synonyms/obsolete |
pck1;
SPAC17G8.14c |
| Gene product |
protein kinase C-like;
Phorbol esters/diacylglercerol binding domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3152 |
| ORF length (spliced) |
2967 |
| Entry clone length |
3152 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
662A:G / 1199T:C /
2708A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22H10.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTGCAGTTGGATGATGC |
| Rev primer name |
SPAC22H10.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCAGTAGCAAAACTGGAA |
| Amino acid length |
988 |
| Molecular weight |
111.7 |
| Isoelectric point (calc.) |
8.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQHLQMRLSI |
| Localization (YFP) |
periphery at site of
septum formation; cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |