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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC688.07c
Gene ID 30/A10
Gene synonyms/obsolete
Gene product serine-rich protein
Entry clone Cloned
ORF length (unspliced) 3117
ORF length (spliced)
Entry clone length 3117
No. of intron 0
Sequence status Finished
Sequence results 1881T:G / 2478A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC688.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGAAAATACGGCTCA
Rev primer name SPAC688.07.Rv
Rev primer SEQ AGAAAGCTGGGTATGATTTGAATCCCAATTTT
Amino acid length 1038
Molecular weight 113.9
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol; periphery at cell tip and site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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