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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3H1.02c
Gene ID 30/A09
Gene synonyms/obsolete
Gene product zinc metallopeptidase; peptidase family M16
Entry clone Cloned
ORF length (unspliced) 3111
ORF length (spliced)
Entry clone length 3111
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3H1.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTTCTGATGACACATC
Rev primer name SPAC3H1.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATTGTCATCATTTCCTCCT
Amino acid length 1036
Molecular weight 117.4
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQQFLEGLKI
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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