| Gene name |
SPAC630.14c |
| Gene ID |
28/H10 |
| Gene synonyms/obsolete |
tup1; tup12 |
| Gene product |
eneral transcriptional
repressor; transcriptional repressor functioning redundantly
with Tup11p; WD repeat protein; histone binding |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2482 |
| ORF length (spliced) |
1761 |
| Entry clone length |
2482 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
416A:G / 1121A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC630.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTACTGTCCGCCAATT |
| Rev primer name |
SPAC630.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAGATCCTCATAAGACCAA |
| Amino acid length |
586 |
| Molecular weight |
64.1 |
| Isoelectric point (calc.) |
6.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol;
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |