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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC12C2.08
Gene ID 28/H09
Gene synonyms/obsolete
Gene product dynamin family; dynamin GTPase effector domain; involved in mitochondrial morphology; involved in mitochondrial localization; involved in mitochondrial fission
Entry clone Cloned
ORF length (unspliced) 2477
ORF length (spliced) 2346
Entry clone length 2477
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC12C2.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACAACTTATTCCATT
Rev primer name SPBC12C2.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAAACCGTTGAAATAATT
Amino acid length 781
Molecular weight 87
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSGRVYPLKL
Localization (YFP) cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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