| Gene name |
SPCC613.04c |
| Gene ID |
28/A06 |
| Gene synonyms/obsolete |
rng3 |
| Gene product |
UCS-domain protein;
involved in cytokinesis; involved in contractile ring assembly
(required); involved in assembly of myosin II containing
proteins at the division site; armadillo repeat protein
(inferred from conntext) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2241 |
| ORF length (spliced) |
|
| Entry clone length |
2241 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC613.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACCCACGAGCTTTCCTC |
| Rev primer name |
SPCC613.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTGCTTCCTTGAAGTCTA |
| Amino acid length |
746 |
| Molecular weight |
84.4 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSTVGIELLI/LSQVIPTLKL/LLNNLSKLLL/LLGKFEVLLAL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
cytosol>nucleus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |