Gene name |
SPAC1687.05 |
Gene ID |
28/A05 |
Gene synonyms/obsolete |
pli1 |
Gene product |
septin interacting
protein homolog; zinc finger protein; zf-MIZ; SAP domain;
DNA-binding protein; septin-sumoylation; possibly interacts
with ubiquitin conjugating enzyme |
Entry clone |
Cloned |
ORF length (unspliced) |
2236 |
ORF length (spliced) |
2184 |
Entry clone length |
2236 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
2134A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1687.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAACCAGGCGAACTTTTT |
Rev primer name |
SPAC1687.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCTATACTCTGAAAAGTG |
Amino acid length |
727 |
Molecular weight |
80.7 |
Isoelectric point (calc.) |
6.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKRLETGLII |
Localization (YFP) |
nucleus; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |