| Gene name |
SPAC3G9.05 |
| Gene ID |
28/A01 |
| Gene synonyms/obsolete |
|
| Gene product |
GIT repeat protein
(SMART); ADP-ribosylation factor; GTPase-activating protein;
involved in cell polarity; involved in conjugation; involved
in conjugation with cellular fusion; predicted coided-coil
region |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2114 |
| ORF length (spliced) |
1980 |
| Entry clone length |
2114 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1856T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC3G9.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAAGTATTATTATTCG |
| Rev primer name |
SPAC3G9.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGGGTGGAGAATTGTGTC |
| Amino acid length |
659 |
| Molecular weight |
73.8 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFDVSSPLEL |
| Localization (YFP) |
periphery at cell tip
and site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |