| Gene name |
SPBC13G1.05 |
| Gene ID |
27/H12 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved eukaryotic
protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2113 |
| ORF length (spliced) |
1950 |
| Entry clone length |
2113 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
591T:C / 1479A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC13G1.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTAGCAATACCTCACC |
| Rev primer name |
SPBC13G1.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCAAATTCTTTTAGAATGC |
| Amino acid length |
649 |
| Molecular weight |
74.7 |
| Isoelectric point (calc.) |
9.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
3 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAYMVLHTLVL/LFFMAIMLKL |
| Localization (YFP) |
ER; Golgi |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |