| Gene name |
SPAC343.05 |
| Gene ID |
27/A06 |
| Gene synonyms/obsolete |
vma1 |
| Gene product |
H(+)-ATPase; vacuolar
ATP synthase (catalytic subunit A) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1957 |
| ORF length (spliced) |
1860 |
| Entry clone length |
1957 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
479G:A / 1078T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC343.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAAGATAATTAGCTGA |
| Rev primer name |
SPAC343.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCAGTCAGAGTGTGAAAC |
| Amino acid length |
619 |
| Molecular weight |
68.8 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMDFPELTI |
| Localization (YFP) |
cytosol; cytoplasmic
dots |
| Comments for localization |
bright large dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |