Gene name |
SPBC18E5.07 |
Gene ID |
26/B10 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical
proline-serine rich protein; low similarity to glucoamylase;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
1848 |
ORF length (spliced) |
|
Entry clone length |
1848 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
319A:G / 344A:G /
1587G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC18E5.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGAAACATCGTCGAA |
Rev primer name |
SPBC18E5.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTGCTCTTCAGTTTCCACT |
Amino acid length |
615 |
Molecular weight |
65.3 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPFDIVSNLDI |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |