| Gene name |
SPAC9.01 |
| Gene ID |
25/H07 |
| Gene synonyms/obsolete |
zwf1;
SPAC3A12.18 |
| Gene product |
glucose-6-phosphate
1-dehydrogenase; involved in pentose-phosphate shunt,
oxidative branch (1st step); involved in glutathione
metabolism; involved in glucose 6-phosphate utilization;
glucose-6-phosphate 1-dehydrogenase activity; similar to Sp
SPAC3C7.13C and SPCC794.01C |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1619 |
| ORF length (spliced) |
1503 |
| Entry clone length |
1619 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
882A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC9.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTCTGCAAATTTGTA |
| Rev primer name |
SPAC9.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTGATCGGAAGGAACGGAC |
| Amino acid length |
500 |
| Molecular weight |
57.2 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |