Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC550.09
Gene ID 25/G10
Gene synonyms/obsolete
Gene product conserved hypothetical; hypothetical protein; dysferlin domain-domain of unknown function present in yeast peroxisomal proteins (SMART)
Entry clone Cloned
ORF length (unspliced) 1608
ORF length (spliced)
Entry clone length 1608
No. of intron 0
Sequence status Finished
Sequence results 341A:G / 572A:G / 575A:G / 833T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC550.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATTCGCGCACATTTGTT
Rev primer name SPCC550.09.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGTGCTTCAGCACCATTA
Amino acid length 535
Molecular weight 60.2
Isoelectric point (calc.) 6.4
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTTLFFTWLII
Localization (YFP) cytoplasmic dots; ambiguous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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