| Gene name |
SPCC550.09 |
| Gene ID |
25/G10 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein; dysferlin domain-domain of
unknown function present in yeast peroxisomal proteins
(SMART) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1608 |
| ORF length (spliced) |
|
| Entry clone length |
1608 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
341A:G / 572A:G /
575A:G / 833T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC550.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTCGCGCACATTTGTT |
| Rev primer name |
SPCC550.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGTGCTTCAGCACCATTA |
| Amino acid length |
535 |
| Molecular weight |
60.2 |
| Isoelectric point (calc.) |
6.4 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTTLFFTWLII |
| Localization (YFP) |
cytoplasmic dots;
ambiguous structure |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |