| Gene name |
SPBC106.13 |
| Gene ID |
23/A11 |
| Gene synonyms/obsolete |
|
| Gene product |
eukaryotic conserved
hypothetical protein; LisH domain; CTLH domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1327 |
| ORF length (spliced) |
1215 |
| Entry clone length |
1327 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
860T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC106.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTTTCGTCCAGAGCA |
| Rev primer name |
SPBC106.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACCACATAAACTTTACGA |
| Amino acid length |
404 |
| Molecular weight |
45.8 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLLLDHLKI/LGGMQSLTL |
| Localization (YFP) |
nucleus>=cytosol;
cytoplasmic dots |
| Comments for localization |
a lot of moving
dots |
| Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol |
| Microscope used for
observation |
DeltaVision |