| Gene name |
SPBC354.01 |
| Gene ID |
22/G02 |
| Gene synonyms/obsolete |
gtp1; SPBC649.06 |
| Gene product |
GTP-binding protein;
GTP1/OBG family; non-essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1305 |
| ORF length (spliced) |
1092 |
| Entry clone length |
1305 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC354.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTGTCTTAGAAAAAGT |
| Rev primer name |
SPBC354.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTGTCACAATAGTAACC |
| Amino acid length |
363 |
| Molecular weight |
40.7 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKERIWEELNL |
| Localization (YFP) |
nucleus>cytosol;
microtubules; spindle microtubules |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |