| Gene name |
SPAC17G6.14c |
| Gene ID |
22/G01 |
| Gene synonyms/obsolete |
uap56 |
| Gene product |
DEAD/DEAH box
helicase; ATP dependent; RNA helicase; involved in mRNA
splicing; involved in mRNA export; TREX complex; involved in
coupling transcription to mRNA export; THO complex
associated |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1305 |
| ORF length (spliced) |
|
| Entry clone length |
1305 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC17G6.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATCTGCTCAGGAGGA |
| Rev primer name |
SPAC17G6.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCATTCATGTATGAGCCA |
| Amino acid length |
434 |
| Molecular weight |
49.2 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
various size of
nucleus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |