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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G6.14c
Gene ID 22/G01
Gene synonyms/obsolete uap56
Gene product DEAD/DEAH box helicase; ATP dependent; RNA helicase; involved in mRNA splicing; involved in mRNA export; TREX complex; involved in coupling transcription to mRNA export; THO complex associated
Entry clone Cloned#
ORF length (unspliced) 1305
ORF length (spliced)
Entry clone length 1305
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC17G6.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCATCTGCTCAGGAGGA
Rev primer name SPAC17G6.14.Rv
Rev primer SEQ AGAAAGCTGGGTATGCATTCATGTATGAGCCA
Amino acid length 434
Molecular weight 49.2
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization various size of nucleus
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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