Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC458.06
Gene ID 22/E09
Gene synonyms/obsolete
Gene product WD repeat protein
Entry clone Cloned
ORF length (unspliced) 1299
ORF length (spliced) 1095
Entry clone length 1299
No. of intron 4
Sequence status Finished
Sequence results 523A:G / 651T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC458.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAACTATTAATACTGT
Rev primer name SPAC458.06.Rv
Rev primer SEQ AGAAAGCTGGGTAGCATAAAGACCCATCTGCC
Amino acid length 364
Molecular weight 41
Isoelectric point (calc.) 8.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYQFGNNLKL
Localization (YFP) Golgi; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.