| Gene name |
SPAC824.09c |
| Gene ID |
22/C07 |
| Gene synonyms/obsolete |
|
| Gene product |
GTPase activating
protein; ArfGap domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1285 |
| ORF length (spliced) |
963 |
| Entry clone length |
1285 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
160A:G / 515A:G /
531A:G / 570C:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC824.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATAAATTCAAAAAGCAA |
| Rev primer name |
SPAC824.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTCCAAACATCATGAGAA |
| Amino acid length |
320 |
| Molecular weight |
35.1 |
| Isoelectric point (calc.) |
10.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
Golgi; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |