Gene name |
SPBC14C8.05c |
Gene ID |
21/A04 |
Gene synonyms/obsolete |
meu17 |
Gene product |
glucoamylase
(glucan-alpha-1,4-glucosidase); glycosyl hydrolase family 15;
sporulation-specific; meiotic expression upregulated |
Entry clone |
Cloned |
ORF length (unspliced) |
1353 |
ORF length (spliced) |
|
Entry clone length |
1353 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
386G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC14C8.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGTACATACTGGTTATT |
Rev primer name |
SPBC14C8.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGACAGAGCCTTTGATTGCC |
Amino acid length |
450 |
Molecular weight |
51.1 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER; Golgi; spore
membrane |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |