Gene name |
SPBC409.19c |
Gene ID |
21/A03 |
Gene synonyms/obsolete |
|
Gene product |
metaxin1; involved in
mitochondrial protein transport; localization preprotein
import complex; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1353 |
ORF length (spliced) |
|
Entry clone length |
1353 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC409.19.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGCAACTTTTCATTTA |
Rev primer name |
SPBC409.19.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACAATCTCTTCCTCCAAG |
Amino acid length |
450 |
Molecular weight |
51.1 |
Isoelectric point (calc.) |
4.3 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEGSVNDLWL |
Localization (YFP) |
nucleus>>cytosol; cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |