| Gene name |
SPCC18.13 |
| Gene ID |
20/G02 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; WD repeat protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1339 |
| ORF length (spliced) |
1266 |
| Entry clone length |
1339 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
711T:deletion /
712T:deletion / 904A:G / 962T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC18.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTGAACAGCGAGTTTT |
| Rev primer name |
SPCC18.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTATCTTTAGATTCAACG |
| Amino acid length |
421 |
| Molecular weight |
47.4 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRSAFSTYLSL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |