| Gene name |
SPAC24B11.06c |
| Gene ID |
16/A05 |
| Gene synonyms/obsolete |
spc1; sty1; phh1 |
| Gene product |
serine/threonine
protein kinase; Spc1 SAPK cascade; interacts physically with
SPBC9B6.10; MAP kinase (MAPK); acts downstream of 2 component
response regulator; involved in mitotic checkpoint; involved
in spindle orientation; involved in oxidative stress
response |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1050 |
| ORF length (spliced) |
|
| Entry clone length |
1050 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC24B11.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGAATTTATTCGTAC |
| Rev primer name |
SPAC24B11.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGATTGCAGTTCATTATCC |
| Amino acid length |
349 |
| Molecular weight |
40.2 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |