| Gene name |
SPBC25H2.14 |
| Gene ID |
16/A04 |
| Gene synonyms/obsolete |
|
| Gene product |
eukaryotic conserved
protein; 5 predicted transmembrane helices; involved in
vesicular transport and membrane fusion from Sc 2-hybrid data
|
| Entry clone |
Cloned |
| ORF length (unspliced) |
1049 |
| ORF length (spliced) |
708 |
| Entry clone length |
1049 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
236A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC25H2.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAGGTCATTTCGTAA |
| Rev primer name |
SPBC25H2.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCACTTTTTCCAAAATAA |
| Amino acid length |
235 |
| Molecular weight |
27.7 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
5 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLYVIQLFL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |