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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1142.06
Gene ID 14/D05
Gene synonyms/obsolete
Gene product ArsA ATPase family; ATPase; involved in anion transport; involved in metal homeostasis
Entry clone Cloned
ORF length (unspliced) 990
ORF length (spliced)
Entry clone length 990
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1142.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCTTCGACCCATTGCC
Rev primer name SPAC1142.06.Rv
Rev primer SEQ AGAAAGCTGGGTATTCTTTGCCAGAAGTAGGA
Amino acid length 329
Molecular weight 36.5
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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