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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC24C9.14
Gene ID 14/D04
Gene synonyms/obsolete
Gene product OTU-like cysteine protease
Entry clone Cloned
ORF length (unspliced) 990
ORF length (spliced)
Entry clone length 990
No. of intron 0
Sequence status Finished
Sequence results 111G:A / 863T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC24C9.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTCACTACGTCTTCG
Rev primer name SPAC24C9.14.Rv
Rev primer SEQ AGAAAGCTGGGTAATACTCACCAAACTGTGTA
Amino acid length 329
Molecular weight 35.6
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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