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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31G5.02
Gene ID 13/H09
Gene synonyms/obsolete
Gene product involved in cell cycle control
Entry clone Cloned
ORF length (unspliced) 974
ORF length (spliced) 699
Entry clone length 974
No. of intron 2
Sequence status Finished
Sequence results 907A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC31G5.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCATCTTCTTTATCAAAT
Rev primer name SPAC31G5.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAGATACTACTAAATACCCA
Amino acid length 232
Molecular weight 26.4
Isoelectric point (calc.) 4.4
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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