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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1B3.01c
Gene ID 13/H08
Gene synonyms/obsolete
Gene product uracil phosphoribosyltransferase
Entry clone Cloned
ORF length (unspliced) 973
ORF length (spliced) 660
Entry clone length 973
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1B3.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGCTGGTACCTGAGCA
Rev primer name SPAC1B3.01.Rv
Rev primer SEQ AGAAAGCTGGGTAGGCCTTCGTTCCAAAGTAA
Amino acid length 219
Molecular weight 24.4
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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