Gene name |
SPBC12C2.07c |
Gene ID |
12/C04 |
Gene synonyms/obsolete |
|
Gene product |
spermidine
synthase |
Entry clone |
Cloned |
ORF length (unspliced) |
897 |
ORF length (spliced) |
|
Entry clone length |
897 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPC12C2.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGTTCAAGAACTTTC |
Rev primer name |
SPC12C2.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGAGGAGGTAGCCTTGTCA |
Amino acid length |
298 |
Molecular weight |
33.1 |
Isoelectric point (calc.) |
5.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |