| Gene name |
SPCC285.08 |
| Gene ID |
12/C03 |
| Gene synonyms/obsolete |
ret2 |
| Gene product |
coatomer (delta
subunit) (putative); Sp gene appears to be truncated
***possible deletion check*** |
| Entry clone |
Cloned |
| ORF length (unspliced) |
895 |
| ORF length (spliced) |
723 |
| Entry clone length |
895 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
572G:deletion |
| Comments |
S. pombe gene appears
to be truncated #possible deletion check |
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC285.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAAGAAGGTCTCATGC |
| Rev primer name |
SPCC285.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATATAACAAGCTAGTATTC |
| Amino acid length |
240 |
| Molecular weight |
27 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQDIDTLHL |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |