Gene name |
SPCC285.08 |
Gene ID |
12/C03 |
Gene synonyms/obsolete |
ret2 |
Gene product |
coatomer (delta
subunit) (putative); Sp gene appears to be truncated
***possible deletion check*** |
Entry clone |
Cloned |
ORF length (unspliced) |
895 |
ORF length (spliced) |
723 |
Entry clone length |
895 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
572G:deletion |
Comments |
S. pombe gene appears
to be truncated #possible deletion check |
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC285.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAAGAAGGTCTCATGC |
Rev primer name |
SPCC285.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATATAACAAGCTAGTATTC |
Amino acid length |
240 |
Molecular weight |
27 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQDIDTLHL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |