Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1B3.06c
Gene ID 10/H06
Gene synonyms/obsolete
Gene product methyltransferase; UbiE family; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 837
ORF length (spliced)
Entry clone length 837
No. of intron 0
Sequence status Finished
Sequence results 445T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1B3.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTAAGCCGACGGATCA
Rev primer name SPAC1B3.06.Rv
Rev primer SEQ AGAAAGCTGGGTATTCCTCTTTTTTATATACG
Amino acid length 278
Molecular weight 31.7
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization aggregates
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.