Gene name |
SPAC1B3.06c |
Gene ID |
10/H06 |
Gene synonyms/obsolete |
|
Gene product |
methyltransferase;
UbiE family; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
837 |
ORF length (spliced) |
|
Entry clone length |
837 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
445T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1B3.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAAGCCGACGGATCA |
Rev primer name |
SPAC1B3.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCCTCTTTTTTATATACG |
Amino acid length |
278 |
Molecular weight |
31.7 |
Isoelectric point (calc.) |
6.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
aggregates |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|