| Gene name |
SPAC1805.02c |
| Gene ID |
10/H05 |
| Gene synonyms/obsolete |
|
| Gene product |
electron transfer
flavoprotein (beta subunit) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
837 |
| ORF length (spliced) |
765 |
| Entry clone length |
837 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1805.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAAAATTCGCATTTT |
| Rev primer name |
SPAC1805.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGAGGGCGCCAAGTTCCTTT |
| Amino acid length |
254 |
| Molecular weight |
27.7 |
| Isoelectric point (calc.) |
10 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPAIITTDLRL |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |