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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC24B10.21
Gene ID 09/C02
Gene synonyms/obsolete tpi1; tpi
Gene product triosephosphate isomerase
Entry clone Cloned
ORF length (unspliced) 750
ORF length (spliced)
Entry clone length 750
No. of intron 0
Sequence status Finished
Sequence results 384T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC24B10.21.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCACGTAAATTCTTTGT
Rev primer name SPCC24B10.21.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGGCTGTGAACATTAACA
Amino acid length 249
Molecular weight 27.2
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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