Gene name |
SPCC24B10.06 |
Gene ID |
09/C01 |
Gene synonyms/obsolete |
|
Gene product |
Sp specific families;
hypothetical protein; GPI anchored protein (pers. comm. Birgit
Eisenhaber); glycoprotein; no apparent Sc ortholog; no
apparent orthologs |
Entry clone |
Cloned# |
ORF length (unspliced) |
750 |
ORF length (spliced) |
471 |
Entry clone length |
750 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC24B10.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTGGAAAAGTTTCATC |
Rev primer name |
SPCC24B10.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGGACTAAAGCGGCAAAA |
Amino acid length |
156 |
Molecular weight |
16.7 |
Isoelectric point (calc.) |
4.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLVFASFLII/LLIVSLLSI |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |