| Gene name |
SPAC29A4.09 |
| Gene ID |
08/C01 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved eukaryotic
protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
699 |
| ORF length (spliced) |
612 |
| Entry clone length |
699 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
67A:G / 299T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC29A4.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAATACATCTCTACT |
| Rev primer name |
SPAC29A4.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGTCTTATTTTCTTCTTT |
| Amino acid length |
203 |
| Molecular weight |
24.3 |
| Isoelectric point (calc.) |
10 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
194/195/58 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>>nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|