Gene name |
SPAC9E9.01 |
Gene ID |
02/A08 |
Gene synonyms/obsolete |
|
Gene product |
very hypothetical
protein; sequence orphan |
Entry clone |
Cloned in 2004
trial |
ORF length (unspliced) |
341 |
ORF length (spliced) |
300 |
Entry clone length |
341 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC9E9.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGGATGGCTTGGAGCT |
Rev primer name |
SPAC9E9.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACCAATTAACCCTTGAAAGG |
Amino acid length |
99 |
Molecular weight |
11.3 |
Isoelectric point (calc.) |
7.4 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
cytosol? |
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
DeltaVision |