| Gene name |
SPBC1677.02 |
| Gene ID |
02/A02 |
| Gene synonyms/obsolete |
dpm3 |
| Gene product |
dolichol phosphate
mannose synthase; can weakly stabilize mammalian DPM1 protein;
no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
338 |
| ORF length (spliced) |
273 |
| Entry clone length |
338 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
151A:G / 154A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1677.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAAGGATTCACAAAGT |
| Rev primer name |
SPBC1677.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTCTTCAATAGTCATACCC |
| Amino acid length |
90 |
| Molecular weight |
10.5 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER? |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |