Photoresponsive substrate for cell culturing


Cell culturing substrates where cell adhesion can be switched by external stimuli during cell cultivation are useful scaffold for tissue engineering, cell-based sensors, and fundamental cell biological studies. Our objective is to develop a new "dynamic substrates" where cell adhesion can be switched by light.

 

patterning

Patterned coculturing of cells on a photoresponsive substrate

 

Reference

(1)

Jun Nakanishi, Tohru Takarada, Kazuo Yamaguchi, Mizuo Maeda, ”Recent advances in cell micropatterning techniques for bioanalytical and biomedical sciences” Ana. Sci., 24, 67-72 (2008).

(2)

Jun Nakanishi, Yukiko Kikuchi, Satoshi Inoue, Kazuo Yamaguchi, Tohru Takarada, Mizuo Maeda, “Spatiotemporal Control of Migration of Single Cells on a Photoactivable Cell Microarray” J. Am. Chem. Soc., 129, 6694-6695 (2007).

(3)

Jun Nakanishi, Yukiko Kikuchi, Tohru Takarada, Hidekazu Nakayama, Kazuo Yamaguchi, Mizuo Maeda, “Spatiotemporal Control of Cell Adhesion on a Self-Assembled Monolayer having a Photocleavable Protecting Group” Anal. Chim. Acta, 578, 100-104 (2006).

(4)

Jun Nakanishi, Tohru Takarada, Shinya Yunoki, Yukiko Kikuchi, Mizuo Maeda, “FRET-based Monitoring of Conformational Change of the β2 Adrenergic Receptor in Living Cells” Biochem. Biophys. Res. Commun., 343, 1191-1196 (2006).

(5)

Jun Nakanishi, Yukiko Kikuchi, Tohru Takarada, Hidekazu Nakayama, Kazuo Yamaguchi, Mizuo Maeda, “Photoactivation of a Substrate for Cell Adhesion under Standard Fluorescence Microscopes” J. Am. Chem. Soc. 126, 16314-16315 (2004). 

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