Detection of gene point mutation using affinity capillary electrophoresis

We have developed an affinity capillary electrophoresis (ACE) for reliable and easy to do DNA mutation analysis. ACE is a sequence-specific DNA separation method based on a difference in affinity with ligand DNA which is combined with polyacrylamide in the capillary. We have realized ACE separation of the wild type and a point mutant of K-ras oncogene codon 12.

Detection of the codon 12 mutation in the K- ras oncogene by ACE analysis

References

(1)	Affinity capillary electrophoretic DNA separation using PEG-oligodeoxyribonucleotide block copolymers: Relationship between peak resolution and affinity strength: Naoki Kanayama, Tohru Takarada, Ayumi Kimura, Hideaki Shibata, Mizuo Maeda, J. Sep. Sci., 31, 837-844, (2008).
(2)	Poly(ethylene glycol)-oligodeoxyribonucleotide block copolymers for affinity capillary electrophoretic separation of single-stranded DNAs with a single-base difference: Naoki Kanayama, Tohru Takarada, Ayumi Kimura, Hideaki Shibata, Mizuo Maeda, Reactive & Functional Polymers, 67, 1373-1380 (2007).
(3)	Affinity capillary electrophoresis of DNA for detection of single-nucleotide polymorphisms and point mutations: Comprehensive study for optimization of the weak affinity, Kae Sato, Miwa Onoguchi, Kazuo Hosokawa, and Mizuo Maeda, J. Chromatogr. A 1111, 120-126, (2006).
(4)	Detection of single-base mutation by affinity capillary electrophoresis using a DNA-polyacrylamide conjugate, Kae Sato, Akira Inoue, Kazuo Hosokawa, Mizuo Maeda, Electrophoresis 26, 3076-3080 (2005).
(5)	Capillary electrophoretic discrimination of single nucleotide polymorphisms using oligodeoxyribonucleotide-polyacrylamide conjugate as a pseudo-immobilized affinity ligand: optimum ligand length predicted by melting temperature values; Keiko Mishima, Tohru Takarada, Mizuo Maeda, Anal. Sci. 21, 25-29 (2005).
(6)	Oligodeoxynucleotide-Modified Capillary for Electrophoretic Separation of Single-Stranded DNAs with a Single-Stranded DNAs with a Single-Base Difference; Takahisa Anada, Masako Ogawa, Hisashi Yokomizo, Yoshihisa Ozaki, Tohru Takarada, Yoshiki Katayama, Mizuo Maeda, Anal. Sci., 29, 73-77 (2003).
	The separation of oligodeoxynucleotides having a single-base difference by affinity capillary electrophoresis using oligodeoxynucleotide-polyacrylamide conjugate; Takahisa Anada, Takumi Arisawa, Yoshihisa Ozaki, Tohru Takarada, Yoshiki Katayama, Mizuo Maeda, Electrophoresis, 23, 2267-2273 (2002).

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