|
To download sequence files, please right-click the click button and choose "save" or "download".
All files are created using a free software "ApE".
Although you can open these files in a simple text format, we recommend to use ApE.
| pREP series (Gateway-incompatible) |
|
Plasmid name
|
Backbone
|
Tag
|
Tag position
|
Promoter
|
Selection marker
|
Sequence
|
| pHFF1 |
pREP1
|
His6-FLAG2
|
5'
|
nmt1
|
LEU2
|
|
| pHFF41 |
pREP41
|
His6-FLAG2
|
5'
|
nmt1*
|
LEU2
|
|
| pHFF81 |
pREP81
|
His6-FLAG2
|
5'
|
nmt1**
|
LEU2
|
|
| pHFF148 |
(pREP)
|
His6-FLAG2
|
5'
|
ADH1
|
LEU2
|
|
| pHFG1 |
pREP1
|
His6-FLAG-GFP(S65C)
|
5'
|
nmt1
|
LEU2
|
|
| pHFG41 |
pREP41
|
His6-FLAG-GFP(S65C)
|
5'
|
nmt1*
|
LEU2
|
|
| pHFG81 |
pREP81
|
His6-FLAG-GFP(S65C)
|
5'
|
nmt1**
|
LEU2
|
|
| pHFG148 |
(pREP)
|
His6-FLAG-GFP(S65C)
|
5'
|
ADH1
|
LEU2
|
|
| pFFH1 |
pREP1
|
FLAG2-His6
|
3'
|
nmt1
|
LEU2
|
|
| pFFH41 |
pREP41
|
FLAG2-His6
|
3'
|
nmt1*
|
LEU2
|
|
| pFFH81 |
pREP81
|
FLAG2-His6
|
3'
|
nmt1**
|
LEU2
|
|
| pFFH148 |
(pREP)
|
FLAG2-His6
|
3'
|
ADH1
|
LEU2
|
|
| pGFH1 |
pREP1
|
GFP(S65C)-FLAG-His6
|
3'
|
nmt1
|
LEU2
|
|
| pGFH41 |
pREP41
|
GFP(S65C)-FLAG-His6
|
3'
|
nmt1*
|
LEU2
|
|
| pGFH81 |
pREP81
|
GFP(S65C)-FLAG-His6
|
3'
|
nmt1**
|
LEU2
|
|
| pGFH148 |
(pREP)
|
GFP(S65C)-FLAG-His6
|
3'
|
ADH1
|
LEU2
|
|
|
|
* Medium strength nmt1 promoter, ** Low strength nmt1 promoter
|
 |
 |
|
